Table 2. Simulation results on non-DHFR proteins.

SCOP	Length	PDB	Mutant	Real Tm	MCPU	Eris	SDM	Native energy
All alpha proteins	66	5CRO	Y26D	54.0	0.878	−6.958	−0.690	−2044.4
	66	5CRO	Y26H	49.5	0.869	−3.425	−0.660	−1908.2
	66	5CRO	Y26L	46.0	0.868	−3.458	0.300	−1891.8
	66	5CRO	WT-5CRO	39.5	0.869	0.000	0.000	−1886.0
	66	5CRO	Y26W	37.5	0.871	−1.200	0.350	−1872.7
All beta proteins	67	1CSP	A46E	48.6	1.015	1.203	0.020	−1624.9
	67	1CSP	E3L	62.7	1.033	2.271	−0.460	−1536.7
	67	1CSP	E3R	69.6	1.023	1.638	−0.650	−1878.0
	67	1CSP	E66L	66.4	1.042	2.510	1.320	−1568.1
	67	1CSP	WT-1CSP	53.6	1.022	0.000	0.000	−1596.3
Alpha and beta proteins (a/b)	109	2TRX	D26I	98.0	1.135	2.847	4.290	−2478.5
	109	2TRX	WT-2TRX	87.0	1.107	0.000	0.000	−2558.4
	109	2TRX	T66L	85.0	1.124	3.540	2.180	−2552.0
	109	2TRX	T77V	82.0	1.124	−0.980	1.960	−2541.9
	109	2TRX	C35A	73.0	1.104	−14.670	−2.040	−2574.7
Alpha and beta proteins (a+b)	125	1RN1	V16A	44.5	0.887	2.033	−1.530	−1934.1
	125	1RN1	V16S	36.9	0.876	3.027	−4.410	−1920.1
	125	1RN1	V78S	34.6	0.878	3.870	−4.450	−1943.8
	125	1RN1	V89S	29.6	0.877	3.414	−4.100	−1904.8
	125	1RN1	WT-1RN1	51.5	0.899	0.000	0.000	−1929.7
				Error number	5	7	8	7
				Error rate	0.313	0.438	0.500	0.438
				r	0.708	−0.053	0.635	−0.348

Error number and error rate describe the number and fraction of mutations not predicted in the correct direction (stabilizing vs. destabilizing)