Fig 9. Effect of azepane-1-carboxilate (ACA) and ethanethiol on the activity of recombinant molinate hydrolase.
Assays were performed with 0.5 mM molinate in 50 mM phosphate buffer at pH 7.4 at 30°C, with a protein concentration of 5 μg.ml-1 in the presence of different concentrations of ACA and ethanethiol. The data points represent the mean values from triplicate assays and the error bars are the corresponding standard deviations.