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. 2015 Apr 23;10(4):e0123430. doi: 10.1371/journal.pone.0123430

Fig 10. Site direct mutagenesis of molinate hydrolase.

Fig 10

Detail view of the active site highlighting (in yellow) the residues that potentially restrict the access to the active site. In order to attempt to engineer the enzyme to degrade bulky thiocarbamates, single-site mutants of Arg187, Phe253 and Phe346 to alanine were produced.