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. 2015 Apr 23;11(4):e1004852. doi: 10.1371/journal.ppat.1004852

Fig 1. Schematic representation of viral genomes used in this study.

Fig 1

Positions of miRNA targets for brain-expressed mir-124 and mosquito-specific mir-1, mir-184, or mir-275 in the ORF and 3’NCR of DEN4 genome are indicated by blue and red boxes, respectively. Gray area represents duplicated, codon optimized DEN4 E/NS1 sequence (nts from 2130 to 2451 of DEN4 genome) encoding 98 amino acids from the C-terminal end of the DEN4 E protein and 7 amino acids from the N-terminal end of the NS1 protein. TM1 and TM2 are transmembrane helixes in the C-terminal anchor region of protein E. Red arrows indicate signalase cleavage sites. Asterisk in the table indicates that miRNA target sequence has been altered using synonymous codons.