Fig 6. Effect of mir-184 and mir-275 co-targeting of DEN4 genome in the ORF and 3’NCR on virus fitness in A. aegypti mosquitoes.

A. aegypti were presented with blood meals containing approximately 7 log₁₀ pfu/mL of indicated recombinant viruses and processed after 14 days. (A) Each point represents virus titer in an individual mosquito (n = 24 mosquitoes per group). Horizontal line represents the mean virus titer for all mosquitoes in the group. The dashed line indicates the limit of assay detection [0.2 log₁₀ pfu/mosquito]. P-values used to compare mean titers of D4 and miRNA targeted viruses were calculated using unpaired one-tailed Student's t-test and adjusted using Bonferroni correction method to account for multiple comparisons. (B) Percentage of mosquitoes that became infected (blue) or developed a disseminated infection (orange) with each virus. Differences in infection and dissemination frequencies were compared between D4 and miRNA targeted viruses using one-tailed Fisher’s exact test, and P-values were adjusted using Bonferroni correction method to account for multiple comparisons.