Fig 2. Phylogenetic relationship of moose HEV to other HEVs.

Mega 5.0 was used to generate phylogenetic trees with 1,000 bootstrap replicas and bootstrap values >70% are indicated. a) Maximum likelihood tree based on the 324nt of the partial HEV RNA dependent RNA polymerase gene, avian and rat HEV were used as outgroups. Samples pd12-1053S (serum) and pd12-1053F (feces) were from the same moose, while pd12-943S (serum) and pd12-943F (feces) were taken from another moose. b) Neighbor joining tree based on ~7 kb nucleotides of in frame HEV ORF1 concatenated with ORF2 (representing full genome analysis); trout HEV was used as outgroup. The length of the bar indicates genetic distance. ▼: Moose HEV