Table 1. Moose HEV PCR primers and probe used in the study.

	Primer name	Primer type	Sequence
qPCR
	HEVF8	Forward	AGGTGGTGGTTGGGGCCCT
	HEVR8	Forward	TGGCGAATGGGTTTGAGGGG
	HEVP8	Forward	CGCCTCGACTCGCAGCCATTTGC
	(FAM probe)
Primers used for HEV typing
First PCR
Pool 1	ISP-4232A	Forward	GCATTTCRGCYTGGAGYAAGAC
Pool 1	ISP-4232B	Forward	GTATATCGGCCTGGAGYAAGAC
Pool 1	ISP-4232E	Forward	GYATATCGGCTTGGAGCAAGAC
Pool 2	EAP-4576F	Reverse	AGYGTRCCRGGCTCACCRGA
Pool 2	EAP-4576E	Reverse	AAYGTACCGGGCTCACCRGA
Semi-nested PCR
Pool 1	ISP-4232A	Forward	GCATTTCRGCYTGGAGYAAGAC
Pool 1	ISP-4232B	Forward	GTATATCGGCCTGGAGYAAGAC
Pool 1	ISP-4232E	Forward	GYATATCGGCTTGGAGCAAGAC
Pool 3	IAP-4561E	Reverse	TCACCAGARTGTTTYTTCCATCG
Pool 3	IAP-4561F	Reverse	TCTCCAGAGTGCTTTTTCCAGCA
Pool 3	IAP-4561M	Reverse	TCACCAGAATGTTTTTTCCAACTG