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. 2015 Apr 23;10(4):e0124597. doi: 10.1371/journal.pone.0124597

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© 2015 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — NPCs were plated at 50,000 cells/well and analyzed using a Vybrant MTT cell proliferation assay kit, as described in the methods section. The samples analyzed included a ZNF804A KD line, a scrambled control line, and an un-processed control (no virus transduction or puromycin selection) generated from an iPSC clone derived from the same subject used for the ZNF804A KD. Samples were analyzed in triplicate and the data are presented as means, +/- standard error. Differences were not statistically significant, although a trend towards significance was found in the comparison between the KD and un-processed control on days 4 and 5 (Student’s t-test, two tailed, p = 0.052 and 0.056, respectively).