. 2015 Apr 23;10(4):e0123618. doi: 10.1371/journal.pone.0123618

Table 1. Summary of 35 Hevea brasiliensis ERF genes tested in response to harvesting stress in bark and latex.

ERF group	Gene	Previous studies (Duan et al. 2013; Piyatrakul et al., 2014)					This work
		Contig	Length (bp)	Gene expression marker in non-tapped trees	Phylogenetic analysis	Predicted miR-targeted ERFs	Number of introns	Cis-acting element-related hormonal pathways present in ERF promoters	Gene expression marker in tapped trees
		Contig	Length (bp)	Gene expression marker in non-tapped trees	Phylogenetic analysis	Predicted miR-targeted ERFs	Number of introns		Harvesting stress	Expression in latex
HbERF-I	HbERF-Ib4	CL1Contig10232	1983	Male flower			0	ET, JA, ABA, IAA, CK, GA
	HbERF-Ib7	CL1Contig11231	1956				0	ET, JA, ABA, CK, GA	T, Eth	High
	HbERF-Ib11	CL13631Contig1	905	Male flower, bark, latex			0	ET, JA, ABA, IAA, CK, GA
HbERF-II	HbERF-IIb2	CL17512Contig1	675	Embryo, leaf	ORA47		0	ET, JA, ABA, CK, GA	T, Eth	High
HbERF-III	HbERF-IIIb1	CL7809Contig2	1450	Latex			0	ET, JA, ABA, IAA, CK, GA	Eth
HbERF-III	HbERF-IIIe1	CL1Contig2578	1434			miR894	0	JA, ABA, IAA, CK, GA
HbERF-IV	HbERF-IVa3	CL10676Contig1	1324	Male flower, latex			0	ET, JA, ABA, CK, GA	T, Eth	High
HbERF-V	HbERF-Va2	CL1Contig20078	467				0	ET, JA, ABA, IAA, CK, GA
HbERF-VI	HbERF-VI1	CL15015Contig1	844	Male flower			0	ET, JA, ABA, IAA, CK, GA	T	High
	HbERF-VI3	CL10440Contig2	1585	Embryo, leaf			0	ET, JA, ABA, IAA, CK, GA	T	High
	HbERF-VI5	CL1Contig16711	1616	Latex		miRn11		ET, JA, ABA, IAA, CK, GA	T, Eth	High
HbERF-VI-L	HbERF-VI-L3	CL932Contig9	1213	Male flower	CRF10		0	ET, JA, ABA, CK, GA	Eth
HbERF-VI-L	HbERF-VI-L4	CL5142Contig2	1918	Male flower			1	ET, JA, ABA, CK, GA	T	High
HbERF-VII	HbERF-VIIa1	CL1Contig8301	1014	Embryo, cotyledon			1	ET, JA, ABA, IAA, CK, GA
	HbERF-VIIa12	CL1Contig1275	1825	Bark, latex	RAP2.12		2	ET, JA, ABA, IAA, CK, GA	T	High
	HbERF-VIIa20	CL1Contig726	1046	Leaf	RAP2.3	miR894	1	ET, JA, ABA, CK, GA	T	High
HbERF-VIII	HbERF-VIIIa4	CL1Contig10902	1126	Latex	ERF3		0	JA, ABA, CK, GA	T
	HbERF-VIIIa8	CL1273Contig1	1009	Latex			0	ET, JA, ABA, CK, GA	T
	HbERF-VIIIa9	CL1273Contig2	1270				0	ET, JA, ABA, CK, GA	T	High
	HbERF-VIIIa10	CL1257Contig1	1017		ERF11		0	ET, JA, ABA, IAA, CK, GA	T, Eth	High
	HbERF-VIIIa12	CL1Contig591	1106				0	ET, JA, ABA, CK, GA	T	High
	HbERF-VIIIa13	CL4149Contig1	945		ERF12		0	ET, JA, ABA, IAA, CK, GA	T, Eth	High
	HbERF-VIIIa14	CL4149Contig2	743				0	ET, JA, ABA, IAA, CK, GA	T, Eth	High
	HbERF-VIIIb1	CL1Contig9348	709		DRNL		0	ET, JA, ABA, IAA, CK, GA, OX	T
HbERF-IX	HbERF-IXa3	CL8461Contig3	695	Latex	AtERF1		0	ET, JA, ABA, CK, GA	T, Eth	High
	HbERF-IXb1	CL6925Contig1	1158	Latex		miR408	0	ET, JA, ABA, IAA, CK, GA	T, Eth	High
	HbERF-IXb2	CL10861Contig1	736				0	ET, JA, ABA, CK, GA
	HbERF-IXb3	CL1054Contig1	1221	Latex	ERF5/MACD1		0	ET, JA, ABA, IAA, CK, GA	T
	HbERF-IXc1	CL108Contig2	887				0	ET, JA, ABA, CK, GA	T	High
	HbERF-IXc4	CL4147Contig3	888	Bark	ERF1		0	ET, JA, ABA, IAA, CK, GA, OX	T, Eth	High
	HbERF-IXc5	CL4147Contig1	934	Bark	ERF1	miR894	0	ET, JA, ABA, CK, GA, OX	T, Eth	High
	HbERF-IXc6	CL6690Contig1	1059		ORA59	miR1511	0	ET, JA, ABA, CK, GA	T, Eth
HbERF-X	HbERF-Xa2	CL98Contig4	1526	Immature female flower	RAP2.6-LIKE		1	ET, JA, ABA, IAA, CK, GA	T
	HbERF-Xa8	CL17010Contig1	737		ABR1	miRn11	1	ET, JA, ABA, CK, GA	T, Eth	High
	HbERF-Xb1	CL6880Contig1	1097		RRTF1		0	ET, JA, ABA, IAA, CK, GA	T, Eth	High

The table summarizes earlier works by Duan et al., 2013; Piyatrakul et al., 2014 and current work. T: tapping, Eth: ethephon. ET: ethylene, JA: jasmonic acid, ABA: abscisic acid, IAA: indole acetic acid, CK: cytokinin, GA: gibberellic acid, OX: oxidative stress.