Table 3. Analysis of the relative transcript accumulation of 8 ERF genes of Hevea brasiliensis from group IX by real-time RT-PCR in the bark of the control (C) and plants subjected to seven different treatments: (W) wounding; (MeJA) methyl jasmonate; (ET) ethylene, either individually or in a combination of treatments (T).
Gene | Treatments | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
W | MeJA | ET | W x MeJA | W x ET | MeJA x ET | W x MeJA x ET | ||||||||
ratio | p-value | ratio | p-value | ratio | p-value | ratio | p-value | ratio | p-value | ratio | p-value | ratio | p-value | |
T/C | T/C | T/C | T/C | T/C | T/C | T/C | ||||||||
HbERF-IXa3 | 2.60 | 0.13 | 1.63 | 0.20 | 0.65 | 0.37 | 2.35 | 0.14 | 9.88 | 0.06 | 7.68 | 0.06 | 124.96 | 0.05 |
HbERF-IXb1 | 0.40 | 0.14 | 2.53 | 0.37 | 8.84 | 0.01 | 0.41 | 0.14 | 0.32 | 0.11 | 4.09 | 0.03 | 30.35 | 0.01 |
HbERF-IXb2 | 0.73 | 0.27 | 0.62 | 0.24 | 5.26 | 0.01 | 0.36 | 0.02 | 0.42 | 0.03 | 1.95 | 0.14 | 12.40 | 0.05 |
HbERF-IXb3 | 0.06 | 0.16 | 0.69 | 0.80 | 0.21 | 0.57 | 0.28 | 0.63 | 0.08 | 0.22 | 1.18 | 0.78 | 0.14 | 0.37 |
HbERF-IXc1 | 0.35 | 0.49 | 2.85 | 0.64 | 0.15 | 0.89 | 1.08 | 0.25 | 3.03 | 0.16 | 0.93 | 0.33 | 32.76 | 0.25 |
HbERF-IXc4 | 38.81 | 0.01 | 53.90 | 0.00 | 306.20 | 0.003 | 38.82 | 0.01 | 396.07 | 0.001 | 1754.05 | 0.000 | 3143.14 | 0.003 |
HbERF-IXc5 | 5.38 | 0.03 | 2.95 | 0.04 | 38.53 | 0.002 | 4.36 | 0.02 | 224.85 | 0.001 | 120.67 | 0.004 | 502.54 | 0.02 |
HbERF-IXc6 | 17.09 | 0.01 | 29.52 | 0.01 | 57.90 | 0.003 | 4.28 | 0.08 | 124.79 | 0.002 | 257.63 | 0.002 | 588.72 | 0.01 |
It was considered as an up-regulation when the ratio was >1.0, and a down-regulation when the ratio was <1.0.