Fig 8. Ctr-mediated EphA2 regulation is ERK dependent.

(A) HUVEC cells were left uninfected or infected with Ctr (MOI-1) for 14 h followed by treatment with DMSO or PI3K inhibitor LY294002 (15 μM) or MAPK inhibitor UO126 (15 μM) for 10 h. After 24 h of total infection, cells were subjected to WB analysis against respective proteins. (B) EphA2 plasmid-transfected HeLa cells were left uninfected or infected with Ctr for 24 h as mentioned above the lanes. The cells were treated with DMSO or UO126 (30 μM) for 3 h or 6 h as described above the lanes and subjected to WB analysis to determine the respective proteins as indicated.