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. 2015 Apr 14;180(2):233–242. doi: 10.1111/cei.12577

Fig 2.

Fig 2

Effect of neutralizing interleukin (IL)-19 in healthy control (HC) peripheral blood mononuclear cell (PBMC) cultures. (a) Neutralization of IL-19 in HC PBMC with polyclonal goat anti-IL-19 antibody at a concentration of 5 μg/ml (n = 4). Goat immunoglobulin (Ig)G isotype served as a negative control. Ratios were calculated by normalizing to the chemokine (C-C motif) ligand 2/monocyte chemoattractant protein 1 (CCL2/MCP-1) concentration in supernatants from untreated cultures. Log-transformed data were analysed with the paired t-test. (b) Neutralization of lipopolysaccharide (LPS)-induced IL-19 at 1 ng/ml in healthy control (HC) peripheral blood mononuclear cells (PBMCs) (n = 3). Goat IgG isotype served as a negative control. Ratios were calculated by normalizing to the CCL2/MCP-1 concentration in supernatants from cultures treated with LPS and isotype IgG. Log-transformed data were analysed with the repeated-measures one-way analysis of variance (anova). Boxes and bars indicate median and interquartile range (IQR). *P < 0·05; **P < 0·01.