Table 1.
Biophysical data for the ILA1 T cell receptor (TCR).
| Peptide | Primary sequence | Half-life (s) | KD eq (μM) | Kon (M−1 s−1) | Koff (s−1) | KD kin (μM) |
|---|---|---|---|---|---|---|
| 3G 18 | ILGKFLHWL | 14·7 | 3·7 | 1·6 × 104 | 4·7 × 10−2 | 2·9 |
| 3G8T 23 | ILGKFLHTL | 14·1 | 4 | 1·9 × 104 | 4·9 × 10−2 | 2·5 |
| 8T 18 | ILAKFLHTL | 7·3 | 27·6 | 4 × 103 | 9·5 × 10−2 | 23·75 |
| ILA 2,18 | ILAKFLHWL | 4·9a | 35·3a | 3·8 × 103a | 1·4 × 10−1a | 36a |
| 4L 24 | ILALFLHWL | 3·5 | 117 | 1·7 × 103 | 2 × 10−1 | 117 |
| 5Y 18,23 | ILAKYLHWL | 2·2 | 242 | 1·3 × 103 | 3·2 × 10−1 | 250 |
| 8E 18 | ILAKFLHEL | n.d. | >500 | n.a. | n.a. | n.a. |
KD = affinity; Kon = association rate; Koff = dissociation rate; n.d. = not determined; n.a. = too fast for reliable measurement. The index peptide is underlined. Variant amino acid residues are indicated in italics and bold type.
a
Average of combined studies.