Fig 2.

Proteinuria and podocyte injury in late stages of acute puromycin aminonucleoside (PAN) nephrosis are greater in Wistar rats than in nude rats, or in Wistar rats depleted of macrophages. (a) Plot of protein concentrations in 24-h urine samples taken at various times after PAN injection from Wistar (Wistar-PAN, n = 8) nude rats (nude-PAN, n = 4) and from Wistar rats whose macrophages were depleted by three intraperitoneal (i.p.) injections of clodronate liposomes at 4, 7 and 10 days after PAN injection (Wistar-PAN-CL, n = 4). Results are expressed as means ± standard error; **P < 0·01 compared with nude-PAN and ^#P < 0·05 compared with Wistar-PAN-CL by analysis of variance (anova) followed by Bonferroni's post-test. (b) Micrographs visualizing anti-synaptopodin antibody labelling of representative glomeruli in frozen sections from the same animals used in the experiment shown in (a), along with sham-injected control animals (control). Frozen sections were obtained from animals killked 12 days after PAN injection.