Fig 3.

Activation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) in Per a 10-stimulated dendritic cells (DCs). The DCs pretreated or not with inhibitor for (a) p38 MAPK or (b) NF-κB pathways and stimulated with active or heat-inactivated Per a 10 were analysed by Western blot. Well 1, immature DCs; 2, inhibitor alone; 3, inhibitor plus active Per a 10; 4, active Per a 10 alone; 5, inhibitor plus heat-inactivated Per a 10; and 6, heat-inactivated Per a 10 alone. (c) Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control and (d) expression of tumour necrosis factor (TNF) receptor-associated factor 6 (TRAF6) in Well 1, immature DCs; 2, heat-inactivated Per a 10; and 3, inhibitor plus heat-inactivated Per a 10.