Table 1.
Putative epoxide hydrolases (EHs) retrieved from the BrMgv02 dataset
| Approach (Mangrove) | Best-hit protein (NCBI Accession No.) | Putative microbial source based on homology (Best hit protein) | Hit-length (aa) | Query-length (bp) | e-value | Amino acid identity (%) | ESTHERc |
|---|---|---|---|---|---|---|---|
| 1 (BrMgv02) | Putative epoxide hydrolase (YP_761108) | Hyphomonas neptunium | 320 | 345 | 7e-48 | 73.04 | XE |
| Epoxide hydrolase (NP_823281) | Streptomyces avermitilis | 328 | 394 | 2e-28 | 48.46a | XE | |
| Epoxide hydrolase (YP_002203910) | Streptomyces sviceus | 330 | 393 | 8e-28 | 47.69a | XE | |
| Epoxide hydrolase (NP_767754) | Bradyrhizobium diazoefficiens | 330 | 345 | 7e-24 | 50a | XE | |
| Epoxide hydrolase (YP_002129963.1) | Phenylobacterium zucineum | 321 | 347 | 4e-23 | 51a | XE | |
| 2 (BrMgv02) | Alpha/beta hydrolase fold protein (WP_008687080.1) | Rhodopirellula sallentina | 319 | 850 | 1e-65 | 40ab | XE |
| Epoxide hydrolase – like protein (YP_003705817.1) | Frankia sp. | 398 | 351 | 4e-32 | 51ab | XE | |
| Alpha/beta hydrolase fold protein (YP_002544714.1) | Truepera radiovictrix | 292 | 504 | 3e-38 | 69b | XE | |
| Alpha/beta hydrolase fold protein (YP_003336243.1) | Streptosporangium roseum | 307 | 1459 | 3e-75 | 55ab | XE | |
| Epoxide hydrolase domain protein (WP_007534516.1) | Rhizobium mesoamericanum | 199 | 345 | 3e-21 | 49ab | XE | |
| Epoxide hydrolase (WP_009025691) | Bradyrhizobium sp. | 334 | 802 | 8e-74 | 46ab | XE | |
| Alpha/beta hydrolase (YP_727726.1) | Rhodospirillum rubrum | 305 | 1039 | 4e-116 | 64b | XE |
a
Putative novel protein based on low amino acid identity (< 60%).
b
Complete genes.
c
Classification based on BLASTX against ESTHER database (Lenfant et al., 2013).
The table was constructed using two different approaches: (1) Whole metagenome sequencing – and BLASTX against the LED; (2) Fosmid library sequencing – and BLASTX against the NCBI using the MG-RAST pipeline.
XE, Block_X, Family Epoxide_hydrolase.