Fig. 1.

Phosphorylated Stat3 interacts with Foxp3 and its expression in Treg cells is required for suppression of fatal colitis. (A) Foxp3 immunoprecipitation from nuclear lysates of sorted CD4⁺Foxp3⁻ and CD4⁺Foxp3⁺ cells followed by western blot analysis for the indicated proteins. The input lanes represent 3–5% of cell equivalents used for Foxp3 immunoprecipitation. (B) Spleen and lymph node cellularity. ** P<0.001; * P<0.01. (C) Examples of colon thickening. (D) Weight loss over time (n = 7). (E) IBD scores derived from histopathologic evaluation of colon and cecum from 8–9 week-old mice. Formalin fixed sections were H&E stained prior to examination (n = 4/group). (F) Representative H&E stained colon sections from 8–9 week old mice (original magnification, 20x).