Skip to main content
. Author manuscript; available in PMC: 2015 Apr 23.
Published in final edited form as: Science. 2009 Oct 1;326(5955):986–991. doi: 10.1126/science.1172702

Fig. 5.

Fig. 5

Stat3-dependent gene expression in Treg cells. (A, B) Expression pattern of Stat3dependent genes potentially contributing to Treg suppressor function. The data represent average of two independent microarray experiments. qPCR analysis of relative expression of indicated genes in YFP-Cre+ Treg cells from indicated mice. The results represent mean and standard deviation of relative expression values for indicated genes over Hprt in two independent experiments using three replicates each. (C) Flow cytometric analysis of CCR6, IL-1R and IL-6R expression on Tregs from indicated mice. (D) ELISA and western blot analysis of amounts of TGF-β1, IL-6, and Ebi3 in supernatants of Stat3-sufficient and -deficient Tregs cultured in presence of IL-2. (E) Ratio of Foxp3+YFP+ to Foxp3+YFP− Tregs in spleen, IEL and LPL populations of indicated mice. (F) CD4+Foxp3− were stimulated with anti-CD3 in the presence of culture supernatants derived from Tregs isolated from indicated mice and the frequency of CD4+IL-17+ cells was assessed by flow cytometry. (G) qPCR analysis of Foxp3- and Stat3-bound chromatin isolated from wild-type Tregs using primer set corresponding to the promoter region of the indicated genes. The housekeeping gene Gmpr was used as a specificity control.