Table 2.
Gs- and Gi-coupled GPCRs expressed in FACS-sorted osteoblasts ranked by abundance (validated by Taqman real-time GPCR Array at Ct number below 30)
| Gene symbol | Gene name | Ct number (18S=11) | Class | Endogenous ligand(s) | Coupling |
|---|---|---|---|---|---|
| S1pr1 | sphingosine-1-phosphate receptor 1 | 17 | A | sphingosine-1-phosphate, sphingosylphosphorylcholine | Gi/Go |
| S1pr2 | sphingosine-1-phosphate receptor 2 | 20 | A | sphingosine-1-phosphate, sphingosylphosphorylcholine | Gs, Gq/G11, G12/G13 |
| Lpar1 | lysophosphatidic acid receptor 1 | 20 | A | lysophosphatidic acid | Gi/Go, Gq/G11, G12/G13 |
| Lpar6 | lysophosphatidic acid receptor 6 | 20 | A | lysophosphatidic acid | Gs, Gi/Go, G12/G13 |
| Ccr1 | chemokine (C-C motif) receptor 1 | 20 | A | CCL15, CCL23 | Gi/Go |
| Ptger4 | prostaglandin E receptor 4 (subtype EP4) | 20 | A | PGE2, PGF2α, PGI2, PGD2 | Gs/Gi/Go |
| Gpr30 | G-protein coupled receptor 30 | 21 | A | 17β-estradiol | Gi/Go, Gs |
| Agtr1a | angiotensin II receptor, type 1a | 21 | A | angiotensin I, angiotensin II, angiotensin III | Gi/Go, Gq/G11 |
| Pth1r | parathyroid hormone 1 receptor | 22 | B | Parathyroid hormone, parathyroid hormone related peptide | Gs, Gq/G11 |
| F2rl2 | coagulation factor II (thrombin) receptor-like 2 | 22 | A | thrombin | Gi/Go, Gq/G11, G12/G13 |
| Gpr1 33 | G protein-coupled receptor 133 | 22 | Adhesion | orphan | Gs |
| Aplnr | Apelin receptor | 23 | A | Apelin-13, apelin-17, apelin-36, [Pyr1]apelin-13 | Gi/Go |
| Lpar4 | lysophosphatidic acid receptor 4 | 24 | A | lysophosphatidic acid | Gs, Gi/Go, Gq/G11, G12/G13 |
| Gabbr1 | gamma-aminobutyric acid (GABA) B receptor, 1 | 25 | C | GABA | Gi/Go |
| Lpar3 | lysophosphatidic acid receptor 3 | 26 | A | lysophosphatidic acid | Gi/Go, Gq/G11 |
| Gpr34 | G protein-coupled receptor 34 | 26 | A | lysophosphatidylserine | Gi/Go |
| Gpr68 | G protein-coupled receptor 68 | 26 | A | protons | Gi/Go, Gq/G11 |
| Cxcr4 | chemokine (C-X-C motif) receptor 4 | 27 | A | stroma cell-derived factor 1 | Gi/Go |
| Ptger2 | prostaglandin E receptor 2 (subtype EP2) | 27 | A | PGE2, PGF2α, PGI2, PGD2 | Gs |
| Cmklr1 | chemokine-like receptor 1 | 27 | A | resolving E1, chemerin | Gi/Go |
| Ccr8 | chemokine (C-C motif) receptor 8 | 27 | A | CCL1, CCL8 | Gi/Go |
| Gpbar1 | G protein-coupled bile acid receptor 1 | 28 | A | lithocholic acid, deoxycholic acid, chenodeoxycholic acid, cholic acid | Gs |
| S1pr3 | sphingosine-1-phosphate receptor 3 | 29 | A | sphingosine-1-phosphate, sphingosylphosphorylcholine | Gi/Go, Gq/G11, G12/G13 |
| Sstr2 | somatostatin receptor 2 | 29 | A | SRIF-14, SRIF-28, CST-17 | Gi/Go, Gq/G11 |
| Adra2a | adrenergic receptor, alpha 2a | 29 | A | adrenaline, noradrenaline | Gi/Go, Gs |
| Gpr84 | G protein-coupled receptor 84 | 29 | A | medium-chain-length fatty acid | Gi/Go |
| Cxcr6 | chemokine (C-X-C motif) receptor 6 | 29 | A | CXCL16 | Gi/Go |