. Author manuscript; available in PMC: 2016 May 1.

Published in final edited form as: Bioorg Med Chem Lett. 2015 Mar 24;25(9):1849–1855. doi: 10.1016/j.bmcl.2015.03.051

TABLE 2.

Binding parameters of CCK1R-based chimeric constructs expressed in CHO cells.

CCK1R-based chimeric receptors	Receptor Abbreviations	GR134056X, IC₅₀ (nM)		GR135470X, IC₅₀ (nM)
CCK1R-based chimeric receptors	Receptor Abbreviations	¹²⁵I-BDZ-1	¹²⁵I-CCK	¹²⁵I-BDZ-1	¹²⁵I-CCK
CCK1R WT	CCK1R	NDB	541.0 ± 139.2	NDB	450.0 ± 104.1
N2.61T	CCK1R TM2	NDB	128.0 ± 13.3^*	875.0 ± 296.2	40.3 ± 5.7^*
T3.28V, T3.29S	CCK1R TM3	259.0 ± 60.0^*	1000 ± 115.5	50.2 ± 6.6^*	333.6 ± 33.1
I6.51V, F6.52Y	CCK1R TM6	>1000	316.7 ± 60.0	>1000	151.0 ± 26.8^*
L7.39H	CCK1R TM7	505.3 ± 97.4^*	303.3 ± 3.3	233.3 ± 66.6^*	153.3 ± 3.3^*

p < 0.05 values compared with wild type CCK1R; NDB - no detectable binding.