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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Methods Cell Biol. 2011;104:151–172. doi: 10.1016/B978-0-12-374814-0.00009-4

Scheme 1. Crosslinker syntheses.

Scheme 1

Synthetic procedures for preparing the propargyl-functionalized, photocleavable crosslinker (A) and azide-functionalized crosslinker (B). TMS = trimethylsilane, Ts = tosyl, DIPEA = diisopropyl ethyl amine, CDI = carbonyl diimidizole, DSC = disuccinimidyl carbonate. Adapted with permission (Ouyang et al., 2009; Copyright 2009, American Chemical Society).