Scheme 1. Crosslinker syntheses.
Synthetic procedures for preparing the propargyl-functionalized, photocleavable crosslinker (A) and azide-functionalized crosslinker (B). TMS = trimethylsilane, Ts = tosyl, DIPEA = diisopropyl ethyl amine, CDI = carbonyl diimidizole, DSC = disuccinimidyl carbonate. Adapted with permission (Ouyang et al., 2009; Copyright 2009, American Chemical Society).