Fig. 5.

Bn-FAE1.1 expression in vegetative tissues. a Reporter gene activity was visualised in tissues of flowering Brassica napus plants harbouring the Bn-pFAE1.1 ₈₃₇ ::GUS construct. A, B Open flower. C, D young expanding leaf. E, F section of primary stem. G primary and secondary roots. H–J germinating seedlings, H cotyledons and hypocotyl, I hypocotyl and primary root at the collet region of the seedling shown in H. J cotyledons, hypocotyl and root tip. K hand cut transverse section of hypocotyl of seedling shown in H. L hand cut transverse section of root of seedling shown in I. M Hand cut transverse section of root shown in J. B, D, F, J, M tissues from non-transformed control plants. b RT-PCR analysis of Bn-FAE1.1 and Bn-FAE1.2 gene expression in tissues of B. napus. Expression of RPL2 controls quantity of RNA template. RT presence or absence of reverse transcriptase, M indicates size marker