Immunohistochemistry staining of AKT (A) and phosphorylated-AKT (B) and integrated optical density of AKT (C) and phosphorylated-AKT (D). From left to right panel: Normal saline (NS) group, D-galactosamine (D-Galn) group, lipopolysaccharide (LPS) group, and fulminant hepatic failure (FHF) group (A and B). The integrated optical density of AKT (C) in the FHF group was not significantly different compared to the NS group, LPS group and D-Galn group The integrated optical density of phosphorylated-AKT (p-AKT) (D) in the FHF was notably increased compared with the NS group but not significantly different compared with the LPS and D-Galn groups (aP < 0.05 vs the NS group, one-way ANOVA Dunnett test; n ≥ 15).