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. 2015 Apr 18;8:234. doi: 10.1186/s13071-015-0842-1

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© Gong et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of anti-glycan natures on monoclonal antibodies. A. The bindings of monoclonal antibodies with SjEA were measured by ELISA and presented as mean ± SEM of the OD values from triplicate wells at different dilutions. B. Comparison of bindings of monoclonal IgGs with SjEA versus with pSjEA by ELISA. t test was performed on mean values obtained from triplicate wells from single experiment with *** as p < 0.001. C. Inhibitory effects on bindings of monoclonal antibodies by egg antigens devoid of proteins. Similar methods were applied as in Figure 1C and D. Data shown were mean ± SEM values from triplicate wells. D. Mock- treated (lane 1) or PNGase F-treated egg antigens (lane 2) were detected by monoclonal antibodies with western blot analysis. HRP-ConA was used to test the effect of PNGase F digestion on SjEA and anti-GST was used as loading control. Shown was a representative of at least three separate experiments.