Figure 7. The peroxiredoxin prdx-2 functions in the I2 neurons for feeding inhibition by light.
(A) prdx-2(gk169) mutants had a defective acute response to light. n = 60 worms.
(B) prdx-2; lite-1 double mutants had a worse defect than prdx-2 single mutants. n = 60 worms.
(C) prdx-2 showed a broad expression pattern, as indicated by a functional prdx-2prom::prdx-2::mCherry transgene with mosaic transmission.
(D) Inset: mCherry-tagged prdx-2 was localized throughout I2. Arrowheads point to two parts of the anterior neurite, the soma, and the posterior neurite. Scale bar = 50 μm (top), 10 μm (middle), 20 μm (inset).
(E) I2-specific prdx-2 expression restored a normal pumping response in prdx-2 mutants. n = 60 worms.
(F) Quantification of the acute response latency to light.
(G) prdx-2 mutants were completely defective in the I2 response to light. n ≥ 20 cells.
(H) I2-specific prdx-2 expression restored the I2 response to light in prdx-2 mutants. n ≥ 20 cells.
(I) Quantification of the peak response of the I2 neurons to light.
(G-I) The posterior neurite of I2 was analyzed.
Shading around traces and error bars indicate s.e.m. *** p < 0.001, t-test compared to the corresponding wild-type or indicated strain.
See also Figures S7 and S11.