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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Nature. 2014 Oct 5;514(7522):322–327. doi: 10.1038/nature13824

Figure 1. Establishment of inducible transposon tagging approach.

Figure 1

a, Transgenic alleles and strategy used for inducible genetic tagging. M2-rtTA, reverse tetracycline-responsive transcriptional activator; HSB, hyperactive Sleeping Beauty transposase; Tn, HSB transposon; STOP, polyadenylation signal; CAGGS, chicken β-actin promoter; TetO, tetracycline-response element. b, Frequency of DsRed+ cells in long-term HSC (LT-HSC), short-term HSC (ST-HSC), multipotent progenitor (MPP), and myeloid progenitors (MyP) in marrow of M2/HSB/Tn mice exposed to Dox for 3 weeks. Shown are representative FACS plots from three independently analysed mice of similar age and induction period. c, Sequence of Tn tags identified from 20 DsRed+ LSK colonies that emerged following methylcellulose culture. gDNA, genomic DNA.