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. 2015 Apr 8;2015:534758. doi: 10.1155/2015/534758

Figure 5.

Figure 5

Knocking-down MRTF-A obstructed angiogenesis and migration in HUVECs by affecting integrin expression. (a) Western blotting confirmed the inhibition of endogenous MRTF-A in HUVECs transfected with siMRTF-A. (b) The capillary-like structures formed by HUVECs were observed on Matrigel. (c) Quantification of the capillary-like structures. ∗∗ P < 0.01, n = 3. The migratory ability of HUVECs transfected with siMRTF-A or control siRNA was determined by wound healing (d) and transwell chamber assays (e). (f) Cell migration was quantified by calculating relative cell numbers. ∗ P < 0.05, n = 3. (g) After transfection with siMRTF-A or control siRNA, HUVECs were treated with VEGF for 24 h, and the expression of integrins α1, α5, and β1 was then estimated by real-time PCR. ∗∗ P < 0.01, n = 3. (h) Western blotting for integrins α1, α5 and β1. (i) The relative quantification of the protein expression. ∗∗ P < 0.01, n = 3.