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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Neurobiol Dis. 2015 Jan 17;76:126–136. doi: 10.1016/j.nbd.2014.12.032

Figure 3. Inflammatory genes are upregulated following pilocarpine-induced SE.

Figure 3

Quantitative real-time PCR (qRT-PCR) was performed to measure the time-course of changes in mRNA levels of a number of inflammatory mediators and markers in mouse hippocampi after pilocarpine-induced SE. These molecules include enzymes: COX-2, mPGES-1, iNOS and NOX-2 (gp91phox); cytokines: IL-1β, IL-6, TNF-α and TGF-β1; chemokines: CCL2, CCL3 and CCL4; EP2 receptor; and gliosis markers: GFAP, S100B, Iba1 and CD11b. All tested genes were substantially induced by SE with COX-2 and IL-1β temporally leading many others (n = 6–8 mice per time point, *P < 0.05; **P < 0.01 compared with control mice, one-way ANOVA and post-hoc Dunnett’s test of the ΔΔCT values from the qRT-PCR). Data are shown as mean ± SEM.