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. 2015 May;21(5):840–850. doi: 10.1261/rna.048116.114

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© 2015 Ivashuta et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Exogenous RNA uptake in WCR. (A) WCR midgut and fat body tissue cultures incubated with labeled long dsRNA and siRNA for 15 h. Both Cy3-labeled long dsRNA (240 bp) and siRNA (21 bp) were taken up by fat body cells, but only Cy3-labeled long dsRNA and not siRNA were internalized by midgut tissue (scale bar, 50 μM). (B) WCR midgut (MG) and fat body (FB) co-cultures Cy3-labeled siRNA were taken up by only fat body tissue but not by midgut tissue (upper panel) (scale bar, 50 μM). Higher magnification of Cy3-labeled siRNA internalization in fat body tissue (lower panel) (scale bar, 5 μM). In both panels, DAPI (blue signal) reveals nuclear DNA staining.