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. 2015 May;21(5):840–850. doi: 10.1261/rna.048116.114

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© 2015 Ivashuta et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Plant-derived siRNA accumulation in insects fed on host plants. (A) Size distribution of host plant sRNAs detected in WCR, CPB, and FAW fed on host plant organs of corn roots, tomato leaves, and corn leaves, respectively. Individual bars represent the percentage of plant-derived siRNA of a certain size to all siRNAs (18–26 nt range) detected in insect larvae. Larvae were fed 11–12 d until third–fourth instars, followed by gut dissection, sRNA sequencing of RNA of carcass tissues and mapping of sRNA reads to corresponding plant genomes. WCR neonates (red) which were not exposed to corn roots were sequenced as a control. sRNA sequencing was done in three replicates and standard deviation bars are shown. (B) Abundance of plant-derived (exogenous) and insect (endogenous) 21-nt long siRNAs in WCR, CPB, and FAW larvae fed on host plants. Error bars show standard deviation.