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. 2015 May;21(5):898–910. doi: 10.1261/rna.045211.114

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© 2015 Kelly and Bedwell; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 1. — A subset of translation and RNA turnover factors are rapidly degraded by autophagy following the onset of nitrogen starvation. (A) Western blots of Rpl3p and Pgk1p, and an RNA gel of rRNA from cells harvested at various times following the onset of nitrogen starvation. Quantitation of Rpl3p (center) and rRNA (right) abundance is also shown. (B) Western blots of translation and RNA turnover factors from a wild-type strain whose abundance did not decrease following a shift to nitrogen starvation (−N). (C) Western blots of translation and RNA turnover factors in wild-type and atg7Δ strains that showed a modest decrease in abundance following a shift to nitrogen starvation (−N). (D) Western blots of translation and RNA turnover factors in wild-type and atg7Δ strains that showed a significant decrease in abundance following a shift to nitrogen starvation (−N). A shift to glucose starvation (−Glu) for the indicated times was also examined. (E) Quantitation of Western blots from D for proteins showing the largest changes in abundance after exposure to nitrogen starvation (−N) for the indicated times (compared with the ribophagy control, Rpl3p). (F) Western blots of eRF3 and eIF4GI in an atg19Δ strain after exposure to nitrogen starvation (−N) for the indicated times. Quantitation is shown to the right. Protein abundance was normalized to Pgk1p from the same extract as an internal control. All experiments were carried out two or more times with similar results. Bar graphs are plotted as mean ± standard deviation.