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. 2015 May;21(5):898–910. doi: 10.1261/rna.045211.114

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© 2015 Kelly and Bedwell; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 7. — Effects of autophagy and Ubp3p on translation following the onset of nitrogen starvation. (A) Polysome analysis on wild-type and atg7Δ strains under conditions of nitrogen excess (left) and nitrogen starvation (right). (P/M) Polysome-to-monosome ratio (where monosome includes the sum of the 40S, 60S, and 80S peaks). (B) Schematic of the dual luciferase readthrough reporters. Each reporter contains either an in-frame nonsense or sense codon between the Renilla and firefly ORFs. (C) Effect of the ubp3Δ mutation on the relative efficiency of translation termination and eRF3 abundance during growth in nitrogen excess. (D) Effect of nitrogen starvation (−N) on the relative efficiency of translation termination in wild-type and atg7Δ strains. All experiments were carried out two or more times with similar results. Bar graphs are plotted as mean ± standard deviation.