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Changes in U6 snRNA conformation throughout the splicing cycle. The relevant region of U6 snRNA is diagrammed in red, U4 in yellow, U2 in purple, and pre-mRNA in gray. Important elements of each RNA structure and the corresponding spliceosomal complexes are labeled. Positions of bases mutated in this study are indicated. Positions of the 5′-splice site (5′-ss) and branch point sequence (BPS) are indicated on the pre-mRNA substrate.
