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. 2015 May;21(5):923–934. doi: 10.1261/rna.048421.114

FIGURE 3.

FIGURE 3.

U6–A91G rescues the synthetic lethality of U4–G14U/U6–A62G without restoring U4/U6 pairing. (A) Solution hybridization of whole-cell RNA. RNA extracted under nondenaturing conditions from strain CJM000 bearing the U4 and U6 alleles listed (WT, wild-type) was hybridized to 32P-labeled DNA oligomers complementary to U1 and U4 or U6 (as indicated) at 37°C for 15 min, then analyzed by 9% nondenaturing polyacrylamide gel electrophoresis at 4°C. Note that the U4/U6 RNA complex has a different mobility when bound to a DNA oligomer complementary to U4 or U6. (B) Quantitative analysis of the fraction of U4 present as U4/U6 and total U4 levels normalized to U1 (average of two biological replicates; error bars indicate range). An asterisk indicates a P-value <0.05 in comparison with wild-type as determined by unpaired t-test. A double asterisk indicates a P-value of <0.05 in comparison with U6–A62G, U4-WT.