FIGURE 6.

Suppression by U6–A91G does not correlate with stabilization of the U6-91/U2-14 base pair in Helix II. (A) Positions of the mutations in the U4/U6 and U2/U6 base-paired regions (see also Fig. 1). Black arrows indicate the substitutions. Blue substitutions confer a cold-sensitive growth defect. (B) Serial dilutions of isolates of yeast strain yJPS628 that contain the indicated alleles of the U4, U6, and U2 genes were grown on YEPD medium at the indicated temperatures. Mutant residues are boxed.