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. 2015 May;21(5):935–945. doi: 10.1261/rna.049080.114

FIGURE 4.

FIGURE 4.

Frameshifting efficiency rises with increased CGA–CGA codon pair dosage. (A) Schematic of constructs made to measure the dose dependence of CGA codon pairs on expression and frameshifting. CGA codon pairs are denoted with the white lines for the (CGA)2(AGA)4 and (CGA)3(AGA)3 constructs, illustrating that addition of a third CGA codon results in two CGA–CGA codon pairs. (B) Exponential decrease in GFP/RFP as function of CGA–CGA codon pair dosage for the in-frame Renilla Luciferase-GFP reporter in wild-type and asc1-Δ strains. (C) Exponential increase in GFP/RFP from the +1 frameshifted Renilla Luciferase-GFP reporter as function of CGA–CGA codon pair dosage in the asc1-Δ strain. Frameshifting is not observed in the wild-type strain. (D) Comparison of frameshifting with a single (CGA)3 triple, with one or two separated (CGA)2 pairs. AGA codons are designated as A and CGA codons are designated as C.