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Staining VSFP2.3-expressing cortex with RH1691. Brightfield images (top row), Citrine fluorescence (middle row) and red fluorescence (bottom row), before (A), after staining with RH1691 (B; 15 min after washing with dye free solution) and the end of the in vivo experiment (C; 240 min after washing). The white-dashed outlines indicate the area of craniotomy. Note that VSFP2.3 fluorescence was strongly diminished by the RH1691 stain. Scale bar, 1 mm.
