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. 2015 Apr 24;9:158. doi: 10.3389/fncel.2015.00158

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Copyright © 2015 Zhao, Ma, Leong, Han, VanDongen, Chen and Goh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of hMeCP2-FL but not hMeCP2-ΔMBD rescued the defective dendritic morphologies of MeCP2-silenced newborn DG neurons in the adult brain. (A) Schematic diagram showing retroviral construct expressing shMeCP2 together with EGFP-tagged hMeCP2-FL or ΔMBD. (B) Representative images and tracings from confocal three-dimensional (3D) reconstruction of dendrites of adult newborn neurons expressing either hMeCP2-FL-shMeCP2 or hMeCP2-ΔMBD-shMeCP2 (Scale bar = 20 μm). (C) Quantification of total dendritic length of hMeCP2-FL-shMeCP2 or hMeCP2-ΔMBD-shMeCP2-expressing adult newborn neurons in DG. Each symbol represents a single neuron. (D) Quantification of total branch number of hMeCP2-FL-shMeCP2 or hMeCP2-ΔMBD-shMeCP2-expressing adult newborn neurons in DG. (***p < 0.001 Student’s t-test, n = 21 neurons for hMeCP2-FL-shMeCP2 and 14 neurons for or hMeCP2-ΔMBD-shMeCP2 groups, from 5 mice per experimental group).