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. 2015 Apr 24;6:188. doi: 10.3389/fimmu.2015.00188

Figure 3.

Figure 3

Cutaneous DENV inoculation induces an increased quantity of GC B cells (IgD−CD19 + PNA+) in DLNs compared to control conditions. (A) Representative flow cytometry plots indicate GC B cells percentage per each experimental condition described. Live IgD−singlets (not shown) were gated on GC (CD19 + PNA+) cells. Mice were i.d. inoculated with DENV, iDENV, C6/36, OVA, or PBS at day 0 and boosted at day 7. At day 14, post-inoculation cell suspensions from DLN were labeled for GC B cells. DENV inoculation induced the biggest percentage (B), and the highest quantity of GC B cells per DLN (C). OVA was used as positive control for GCs induction. Data shown are pooled from three to six independent experiments, represent the mean ± SEM, and were analyzed with unpaired two-tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001.