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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biochem J. 2015 Apr 1;467(1):115–126. doi: 10.1042/BJ20141018

Figure 2. Overexpression of OGT or OGA does not affect bioenergetic reserve.

Figure 2

NRCMs were subjected to adenoviral overexpression of OGT and OGA. (A) Immunoblot for OGT and OGA protein expression following adenoviral treatment. (B) Immunoblot for protein O-GlcNAcylation demonstrated an induction of O-GlcNAc in response to Ad-OGT and a reduction in response to Ad-OGA. (C) Mitochondrial function assay of NRCMs 48 h post-transfection. Parameters of mitochondrial function were calculated from (C), including: (D) basal respiration, (E) maximal respiration, (F) reserve capacity, (G) ATP-linked respiration, and (H) proton leak. As indicated in bars, n = 5 independent experiments; *P < 0.05, compared with Ad-Null.