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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Biochem J. 2015 Apr 1;467(1):115–126. doi: 10.1042/BJ20141018

Figure 3. Inhibition of OGA has negligible effects on mitochondrial function.

Figure 3

NRCMs were treated with TMG (1 µM) for 48 h to induce protein O-GlcNAcylation. (A) Immunoblot for whole-cell protein O-GlcNAcylation. (B) Densitometric measurement of O-GlcNAcylation. (C) Immunoblot for mitochondrial protein O-GlcNAcylation following treatment with 1 µM TMG. (D) Mitochondrial function assay of NRCMs following 48 h treatment with TMG. Parameters of mitochondrial function were measured from (D), including: (E) basal respiration, (F) maximal respiration, (G) reserve capacity, (H) ATP-linked respiration, and (I) proton leak. As indicated in bars, n = 6 independent experiments; *P < 0.05.