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. Author manuscript; available in PMC: 2015 Apr 24.
Published in final edited form as: Gene Ther. 1999 Jun;6(6):1006–1014. doi: 10.1038/sj.gt.3300924

Figure 6.

Figure 6

The presence of nuclear targeting sequences increases gene expression in transfected cells in a cell-specific manner. (a) CV1 cells were arrested in G1 by 24 h pretreatment with 50 μm aphidicolin and transfected with lipofectin. Wells of a 12-well dish received 3 μl of lipofectin complexed with 1 μg of plasmid. Aphidicolin remained present throughout the experiment. Luciferase activities from quadruplicate wells were measured in lysates prepared at 48 h after transfection and normalized to total protein. (b) Differentiated human SMCs were transfected with DEAE-dextran as described in Materials and methods. Luciferase activities from quadruplicate wells were measured as in (a). These results are representative of at least three independent experiments.