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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Mol Nutr Food Res. 2015 Mar 11;59(4):622–633. doi: 10.1002/mnfr.201400631

Figure 7. C and Q prevent TNFα-induced PPARγ downregulation in 3T3-L1 adipocytes.

Figure 7

3T3-L1 adipocytes were incubated without or with catechin (C), quercetin (Q) or both compounds (CQ) (1–10 µM) for 4 h, and subsequently in the absence or presence of 20 ng/ml TNFα for further 2 h (PPARγ-DNA binding) or 24 h (PPARγ). (A) PPAR-DNA binding in nuclear fractions as determined by EMSA, and (B) nuclear PPARγ protein levels referred to the nuclear content of heterogeneous nuclear ribonucleoprotein (hRNP). (A and B) Bands were quantified and results were referred to untreated cell values (Arbitrary unit = 1). Results are shown as mean ± SEM of three independent experiments. Values having different superscripts are significantly different, p < 0.05, one way ANOVA test.