| PCR 1 (specific) | PCR 2 (universal) |
| empty gRNA vector (10 ng) | empty gRNA vector(10 ng) |
| gRNAFISceGFP (specific) primer (200 nM) | gRNAF2 (universal) primer (200 nM) |
| gRNAR2 (universal) primer (200 nM) | gRNARI (universal) primer (200 nM) |
| dNTP (200 nM) | dNTP (200 nM) |
| 10× reaction buffer (5 μL) | 10× reaction buffer (5 μL) |
| H20 (fill up to 25 μL) | H20 (fill up to 25 μL) |
| iProof polymerase (0.2 μL) | iProof polymerase (0.2 μL) |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.