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Optimized nucleofection conditions for tested cell lines.
| Cell line | Nucleofector program |
DNA quantity (μg) Cas9:gRNA:DR-GFP |
|---|---|---|
| HEK293 | A-023 | 1:1:2 |
| U20S | X-001 | 1:1:1 or 1:1:2 |
| AA8 (Chinese hamster cells) | D-023 | 5:2.5:5 |
| mouse embryonic stem cells | A-023 | 4:4:4 (maximum cell survival) or 15:5:5 (maximum transfection efficiency) |
