Table 2.
Plasmid mixes for the experiment described in Fig. 2 (DNA quantity in μg in parentheses). To equalize the total amount of DNA in each sample, an empty plasmid (pCAGGS) is added to samples 6 and 7.
| Sample 1 | Sample 2 | Sample 3 | Sample 4 | Sample 5 | Sample 6 | Sample 7 | |
|---|---|---|---|---|---|---|---|
| Cas9 | WT (1) |
WT (1) |
D10A (1) |
H840A (1) |
D10A/ H840A (1) |
- | - |
| gRNA | empty (1) |
SceGFP (1) |
SceGFP (1) |
SceGFP (1) |
SceGFP (1) |
- | - |
| DR-GFP | (2) | (2) | (2) | (2) | (2) | (2) | - |
| Other | pCBAScel (1) + pCAGGS (1) |
NZE-GFP (2) + pCAGGS (2) |