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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Health Innov Point Care Conf. 2014 Oct;2014:10–13. doi: 10.1109/HIC.2014.7038862

Figure 4.

Figure 4

The optical system consists of three lenses and various other components: from left to right, image surface, coverslip glass, lens 1, aperture stop, glued achromatic doublet, emission filter, camera IR filter, and the image surface. Our system was achromatized for two wavelengths, 525 nm and 650 nm, corresponding to the emission peaks of acridine orange bound to DNA and RNA, respectively. In order to simplify optical layout, our system was designed with a curved image surface (radii of curvature: 40 mm). Image surface sag at the end of the field of view equals 63 µm and is below depth-of-focus of our system (DOF = 107 µm). A DOF larger than an image surface sag guarantees that sample will be sharply imaged on the plane surface of image detector. The entire optical system length is 77 mm.