Fig. 5.

Interaction of different integrin ligands with cells expressing or not the α9/p75^NTR complex in a cell adhesion assay. (A) Adhesion of the LBC3 cell line transfectants to immobilized VCAM-1, NGF, VLO5 and collagen I. Integrin ligands were immobilized on 96-well plate by overnight incubation in PBS at 4°C. Adhesion with CMFDA-labeled cells was performed as described in Material and Methods. (B) Inhibition of adhesion of LBC3^α9+/p75+ and LBC3^α9+ cell lines to immobilized VCAM-1 (3 μg/ml) and NGF (10 μg/ml) by VLO5. VLO5 was pre-incubated with CMFDA-labeled cells prior to addition to the wells with immobilized ligands. (*) p<0.001 for LBC3^α9+/p75+ in comparison with LBC3^α9+ cell lines. The error bars represent the standard deviation from three independent experiments.