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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cell Signal. 2015 Mar 5;27(6):1225–1236. doi: 10.1016/j.cellsig.2015.02.029

Fig. 5.

Fig. 5

Interaction of different integrin ligands with cells expressing or not the α9/p75NTR complex in a cell adhesion assay. (A) Adhesion of the LBC3 cell line transfectants to immobilized VCAM-1, NGF, VLO5 and collagen I. Integrin ligands were immobilized on 96-well plate by overnight incubation in PBS at 4°C. Adhesion with CMFDA-labeled cells was performed as described in Material and Methods. (B) Inhibition of adhesion of LBC3α9+/p75+ and LBC3α9+ cell lines to immobilized VCAM-1 (3 μg/ml) and NGF (10 μg/ml) by VLO5. VLO5 was pre-incubated with CMFDA-labeled cells prior to addition to the wells with immobilized ligands. (*) p<0.001 for LBC3α9+/p75+ in comparison with LBC3α9+ cell lines. The error bars represent the standard deviation from three independent experiments.