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. 2014 Dec 2;73(5):390–401. doi: 10.1111/aji.12341

Figure 1.

Figure 1

IgG purified from patients with VT−/PM+ APS inhibit HTR-8 cell invasion, which is abrogated when cells are treated with a TLR4 inhibitor. The ability of HTR-8 cells to invade after treatment with 100 μg/mL pooled IgG from VT+/PM− with 83.7 GPLU and 66.0SU binding activity, VT−/PM+ with 47.2GPLU and 63.8SU binding activity and HCs with 0GPLU and SU binding (a) and following pre-treatment with the TLR4 inhibitor CLI-095 or TLR4 antagonist, Ultra Pure Rhodobacter sphaeroides LPS (b) was measured using a transwell invasion assay after 48 hr. HC cell invasion was set at 100%, and the relative invasion of HTR-8 cells exposed to APS-IgG was analysed from this. Graph shows mean ± SEM of quantitative analysis from six (a) and three (b) independent experiments. Statistical analysis was performed as follows: (a) one-way anova (P = 0.01) with Dunn's multiple comparisons test (*P < 0.05); (b) one-way anova (P = 0.03) with Dunn's multiple comparisons test (*P < 0.05).