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. 2014 Dec 2;73(5):390–401. doi: 10.1111/aji.12341

Figure 2.

Figure 2

HTR-8 cells treated with VT−/PM+ IgG but not HTR-8 cells treated with VT+/PM− IgG increase TLR4 and TRIF transcript levels. HTR-8 cells were treated with 100 μg/mL pooled IgG from VT+/PM− with 78.2GPLU and 44.4SU binding activity, VT−/PM+ with 83.4GPLU and 71.5SU binding activity and HCs with 0GPLU and SU binding. TLR4 (a), TRIF (b) and MyD88 (c) mRNA expression at 6 hr was measured by qRT-PCR. TRIF mRNA expression in HTR-8 cells pre-treatment with the TLR4 inhibitor CLI-095 before treatment with 100 μg/mL pooled IgG from VT+/PM−, VT−/PM+ and HCs was also measured (d). The mean ± SEM of quantitative analysis from four independent experiments is shown. Results are expressed as fold change of either TLR4, TRIF or MyD88/GAPDH, relative to untreated cells. No statistically significant differences were found by one-way anova.